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Fig. 2. Accumulation of synthetic lipofuscin in cultured cells. Midpassage human WI-38 fibroblasts were cultured with (lower panel) or without (upper panel) UV-cross-linked mouse liver mitochondria in the culture media. After two weeks of culture, autofluorescent granules could be detected in the cytoplasm of treated cells. In this experiment, autofluorescence was detected using a rhodamine filter cube. Nuclei were counterstained with a fluorescent dye that reveals cell nuclei (DAPI, blue). Scale bars, 50 µm.
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