Sci. Aging Knowl. Environ., 14 November 2001
A DNA Microarray-Based Genetic Screen for Nonhomologous End Joining Mutants in Saccharomyces cerevisiae
Siew Loon Ooi, Daniel D. Shoemaker, and Jef D. Boekehttp://sageke.sciencemag.org/cgi/content/abstract/sageke;2001/7/or24
Abstract: Science 8 November 2001 (10.1126/science.1065672).
We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-oligomer oligonucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the N-terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.
Science of Aging Knowledge Environment. ISSN 1539-6150