Sci. Aging Knowl. Environ., 12 November 2003
Yeast Life Span Extension by Calorie Restriction Is Independent of NAD Fluctuation
Rozalyn M. Anderson, Magda Latorre-Esteves, Ana Rute Neves, Siva Lavu, Oliver Medvedik, Christopher Taylor, Konrad T. Howitz, Helena Santos, and David A. Sinclairhttp://sageke.sciencemag.org/cgi/content/abstract/sageke;2003/45/or24
Abstract: Science 6 November 2003 (10.1126/science.1088697) (Science Express Reports)
Calorie restriction (CR) slows aging in numerous species. In the yeast S. cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homolog SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.
Science of Aging Knowledge Environment. ISSN 1539-6150