Sci. Aging Knowl. Environ., 24 March 2004
Vol. 2004, Issue 12, p. or5


Genetic Dissection of Complex Traits with Chromosome Substitution Strains of Mice

Jonathan B. Singer, Annie E. Hill, Lindsay C. Burrage, Keith R. Olszens, Junghan Song, Monica Justice, William E. O'Brien, David V. Conti, John S. Witte, Eric S. Lander, and Joseph H. Nadeau

Abstract: Science 18 March 2004 (10.1126/science.1093139) (Science Express Reports)

Chromosome Substitution Strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate detection and identification of genes that control the extraordinary diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.

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