Sci. Aging Knowl. Environ., 18 September 2002
Vol. 2002, Issue 37, p. cp16

CLASSIC PAPERS

Detection of Inactive Enzyme Molecules in Ageing Organisms

Harriet Gershon, and David Gershon

http://sageke.sciencemag.org/cgi/content/abstract/sageke;2002/37/cp16

Abstract: Using immunochemical techniques, evidence is obtained that older nematodes (Turbatrix aceti) contain two populations of enzyme molecules--one active and the other totally inactive.

The synthesis and accumulation of non-functional protein molecules may play a significant part in senescence and the eventual death of organisms. In this communication we report the use of immunological techniques to look directly for the presence of catalytically altered or inactive enzyme molecules in ageing nematodes. The immunological approach is both specific and sensitive and allows for the expression of results in units of catalytic activity per unit of antigenic activity, thus allowing for the detection of partially or totally inactive enzyme molecules present in the form of cross reacting material. This approach has been successfully used in detecting cross reacting protein in bacterial and fungal mutants lacking specific enzyme activities arising from single amino-acid changes caused by point mutations.

Reproduced by permission.

Harriet Gershon, David Gershon, Detection of Inactive Enzyme Molecules in Ageing Organisms. Nature 227, 1214-1217 (1970).

Citation: H. Gershon, D. Gershon, Detection of Inactive Enzyme Molecules in Ageing Organisms. Science's SAGE KE (18 September 2002), http://sageke.sciencemag.org/cgi/content/abstract/sageke;2002/37/cp16








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