SAGE KE Bulletin Board

Eukarion antioxidant efficacy in Flies

12 December 2002

Simon Melov

Florian, Given the negative studies published by Sohal, and other unpublished studies whic also attempted to feed compound to the flies, I dont see the merit in pursuing this line of investigation. As stated in my previous post, the vast majority of studies are with mammals, and this is where we are concentrating our efforts, although we do have some ongoing studies with C.elegans.

The answer to your second question is yes, we do have to inject the mice with the compounds throughout their life (3 x weekly).

You are incorrect with regard to genetic background. Most of our studies in SOD2 nullizygous mice are on a CD1 genetic background (1). We have not tested the compounds for rescue of embryonic lethality in C57Bl6. However in the hybrid mice recently characterized by Ting-Ting Huang and collegues (2), the compounds work as well as they do in the CD-1 background - no better or worse (Hinerfeld et al., unpublished).

1. Melov, S. Animal models of oxidative stress, aging, and therapeutic antioxidant interventions. Int J Biochem Cell Biol 34, 1395-400 (2002).

2. Huang, T.T. et al. Genetic modification of prenatal lethality and dilated cardiomyopathy in Mn superoxide dismutase mutant mice. Free Radic Biol Med 31, 1101-10 (2001).

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